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. 2013:2013:178725.
doi: 10.1155/2013/178725. Epub 2013 Dec 22.

Short communication: subtyping of Mycobacterium kansasii by PCR-restriction enzyme analysis of the hsp65 gene

Zofia Bakuła  1 , Aleksandra Safianowska  2 , Magdalena Nowacka-Mazurek  3 , Jacek Bielecki  1 , Tomasz Jagielski  1
Affiliations

Short communication: subtyping of Mycobacterium kansasii by PCR-restriction enzyme analysis of the hsp65 gene

Zofia Bakuła et al. Biomed Res Int. 2013.

Abstract

Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates of M. kansasii recovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis (PCR-REA) of partial hsp65 gene. Mycobacterium kansasii subtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation of M. kansasii subtype I from clinical specimens may be indicative of infection but may also merely represent colonization.

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Figures

Figure 1
Figure 1
Differentiation of M. kansasii subtypes by PCR-REA of hsp65. Amplified hsp65 fragments were digested with HaeIII (lanes 1–4) and BstEII (lanes 5–8). Lanes: M: GeneRuler 100 bp DNA Ladder (ThermoScienific), ND: nondigested fragment of hsp65.
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References

    1. Hoefsloot W, van Ingen J, Andrejak C, et al. The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: a NTM-NET collaborative study. The European Respiratory Journal. 2013;42(6):604–613. - PubMed
    1. Murai A, Maruyama S, Nagata M, Yuki M. Mastitis caused by Mycobacterium kansasii infection in a dog. Veterinary Clinical Pathology. 2013;42(3):377–381. - PubMed
    1. Amorim A, MacEdo R, Lopes A, Rodrigues I, Pereira E. Non-tuberculous mycobacteria in HIV-negative patients with pulmonary disease in Lisbon, Portugal. Scandinavian Journal of Infectious Diseases. 2010;42(8):626–628. - PubMed
    1. Griffith DE, Aksamit T, Brown-Elliott BA, et al. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. American Journal of Respiratory and Critical Care Medicine. 2007;175(4):367–416. - PubMed
    1. Braun E, Sprecher H, Davidson S, Kassis I. Epidemiology and clinical significance of non-tuberculous mycobacteria isolated from pulmonary specimens. The International Journal of Tuberculosis and Lung Diseases. 2013;17(1):96–99. - PubMed

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