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. 2013:2013:527570.
doi: 10.1155/2013/527570. Epub 2013 Dec 24.

Modes of inhibition of α -amylase and α -glucosidase by aqueous extract of Morinda lucida Benth leaf

M I Kazeem  1 , J O Adamson  1 , I A Ogunwande  2
Affiliations

Modes of inhibition of α -amylase and α -glucosidase by aqueous extract of Morinda lucida Benth leaf

M I Kazeem et al. Biomed Res Int. 2013.

Abstract

Diabetes mellitus is a metabolic disorder of glucose metabolism. The management of blood glucose level is the hallmark in the treatment of this disease. This may be achieved through the use of oral hypoglycemic drugs such as biguanides, insulin secretagogues, and α-glucosidase inhibitors. The purpose of the present study was to investigate the inhibitory effect of Morinda lucida leaf extracts on the activities of α-amylase and α-glucosidase. This was performed using α-amylase from Aspergillus oryzae and α-glucosidase from Saccharomyces cerevisiae. Aqueous extract of Morinda lucida gave the highest percentage yield (9.99%) of the plant out of the three extracts (compared to acetone and ethanolic extracts) and possesses the highest inhibitory activity against α-amylase (IC50 value of 2.30 mg/mL) and α-glucosidase (IC50 value of 2.00 mg/mL). Kinetic analysis revealed that the aqueous extract of this plant leaf inhibited the α-amylase competitively but displayed mixed noncompetitive mode of inhibition towards α-glucosidase. It can be concluded that aqueous extract of Morinda lucida exhibited the best inhibitory activity on the two enzymes studied and the presence of phytochemicals like flavonoids, saponins, and tannins may have contributed greatly to the inhibitory activity of the plant extract.

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Figures

Figure 1
Figure 1
Inhibitory potency of M. lucida extract against α-amylase activity. The values are expressed as means ± SEM of triplicate tests. Means not sharing a common letter at the same concentration were significantly different (P < 0.05).
Figure 2
Figure 2
Mode of inhibition of α-amylase by aqueous extract of M. lucida. (a) Michaelis-Menten plot and (b) Lineweaver-Burk plot.
Figure 3
Figure 3
Inhibitory potency of M. lucida extract against α-glucosidase activity. The values are expressed as means ± SEM of triplicate tests. Means not sharing a common letter at the same concentration were significantly different (P < 0.05).
Figure 4
Figure 4
Mode of inhibition of α-glucosidase by aqueous extract of M. lucida. (a) Michaelis-Menten plot and (b) Lineweaver-Burk plot.
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