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. 2013:2013:946432.
doi: 10.1155/2013/946432. Epub 2013 Dec 17.

Effects of acute exercise and chronic exercise on the liver leptin-AMPK-ACC signaling pathway in rats with type 2 diabetes

Xuejie Yi  1 , Shicheng Cao  2 , Bo Chang  3 , Dalin Zhao  3 , Haining Gao  3 , Yihan Wan  3 , Jiaojiao Shi  3 , Wei Wei  3 , Yifu Guan  4
Affiliations

Effects of acute exercise and chronic exercise on the liver leptin-AMPK-ACC signaling pathway in rats with type 2 diabetes

Xuejie Yi et al. J Diabetes Res. 2013.

Abstract

Aim: To investigate the effects of acute and chronic exercise on glucose and lipid metabolism in liver of rats with type 2 diabetes caused by a high fat diet and low dose streptozotocin (STZ).

Methods: Animals were classified into control (CON), diabetes (DC), diabetic chronic exercise (DCE), and diabetic acute exercise (DAE) groups.

Results: Compared to CON, the leptin levels in serum and liver and ACC phosphorylation were significantly higher in DC, but the levels of liver leptin receptor, AMPK α 1/2, AMPK α 1, and ACC proteins expression and phosphorylation were significantly lower in DC. In addition, the levels of liver glycogen reduced significantly, and the levels of TG and FFA increased significantly in DC compared to CON. Compared to DC, the levels of liver AMPK α 1/2, AMPK α 2, AMPK α 1, and ACC phosphorylation significantly increased in DCE and DAE. However, significant increase of the level of liver leptin receptor and glycogen as well as significant decrease of the level of TG and FFA were observed only in DEC.

Conclusion: Our study demonstrated that both acute and chronic exercise indirectly activated the leptin-AMPK-ACC signaling pathway and increased insulin sensitivity in the liver of type 2 diabetic rats. However, only chronic and long-term exercise improved glucose and lipid metabolism of the liver.

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Figures

Figure 1
Figure 1
Histograph showing the effects of exercise on the leptin-AMPK-ACC pathway in type 2 diabetes rats. The relative levels of protein phosphorylation and protein expression related to the leptin-AMPK-ACC signaling pathway on different groups were shown. (a) Leptin and its receptor. (b) AMPK1/2 and p-AMPK1/2 (Thr172). (c) AMPKα1 and p-AMPKα1 (Thr172). (d) AMPKα2 and p-AMPKα2 (Thr172). (e) ACC and p-ACC (Ser79). Note: in comparison with the control group: **P < 0.01, *P < 0.05; in comparison with the diabetes group: ## P < 0.01, # P < 0.05 (n = 8).
Figure 2
Figure 2
Western blotting images of protein expression and protein phosphorylation related to the leptin-AMPK-ACC signaling pathway of different groups of type 2 diabetes rats. (a) Leptin and its receptor. (b) AMPKα1/2 and p-AMPKα1/2. (c) AMPKα1 and p-AMPKα1. (d) AMPKα2 and p-AMPKα2. (e) ACC and p-ACC. (f) GADPH as an internal standard.
See this image and copyright information in PMC

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