Amylase gene duplication: an ancestral trait in the Drosophila melanogaster species subgroup

Abstract

Electrophoretic polymorphism of amylases was studied in 45 geographic populations of the two cosmopolitan sibling species, D. melanogaster and D. simulans, and in one to three populations or strains of six other species in the D. melanogaster subgroup. Two species, D. erecta and D. orena, for which only a few strains were available were monomorphic. In the other species 2 or 3 amylase variants were identified while in D. melanogaster, 12 electrophoretic variants were characterized. Altogether 17 different amylase isozymes have been observed. The contrast in the level of polymorphism between D. melanogaster and the other species cannot be explained simply by the occurrence of a duplication in the former species. Genetic analysis demonstrated the existence of a duplication in at least 4 other species, namely D. simulans, D. mauritiana, D. yakuba, and D. teissieri. It is therefore suggested that the duplication occurred in a common ancestor and the phylogenetic implications of these observations are discussed.