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Comparative Study
. 2014 Apr;31(4):435-42.
doi: 10.1007/s10815-014-0178-3. Epub 2014 Jan 24.

Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: a time-lapse study

Mette Warming Joergensen  1 , Inge Agerholm, Johnny Hindkjaer, Lars Bolund, Lone Sunde, Hans Jakob Ingerslev, Kirstine Kirkegaard
Affiliations
Comparative Study

Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: a time-lapse study

Mette Warming Joergensen et al. J Assist Reprod Genet. 2014 Apr.

Abstract

Purpose: To analyze the cleavage patterns in dipronuclear (2PN) and tripronuclear (3PN) embryos in relation to fertilization method.

Method: Time-lapse analysis.

Results: Compared to 2PN, more 3PN IVF embryos displayed early cleavage into 3 cells (p < 0.001), displayed longer duration of the 3-cell stage (p < 0.001), and arrested development from the compaction stage and onwards (p < 0.001). For the IVF embryos, the 2nd and 3rd cleavage cycles were completed within the expected time frame. However, timing of the cell divisions within the cleavage cycles differed between the two groups. In contrast, the completion of the 1st, 2nd, and 3rd cleavage cycle was delayed, but with a similar division pattern for 3PN ICSI compared with the 2PN ICSI embryos. 3PN, more often than 2PN ICSI embryos, displayed early cleavage into 3 cells (p = 0.03) and arrested development from the compaction stage and onwards (p = 0.001). More 3PN IVF than ICSI embryos displayed early cleavage into 3 cells (p < 0.001).

Conclusions: This study reports differences in cleavage patterns between 2PN and 3PN embryos and for the first time demonstrates differences in the cleavage pattern between 3PN IVF and ICSI embryos.

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Figures

Fig. 1
Fig. 1
The differences between timing and duration of the cell stages for 3PN IVF (a) and 3PN ICSI (b) compared to their respective 2PN controls. Time-points are reported as medians marked with interquartile range. Wilcoxon rank-sum test was used to test the hypothesis of no difference between timing of events in the 3PN embryos and the control embryos. Significant differences (p < 0.05) between 3 PN embryos and the 2 PN embryos are marked with an asterisk
Fig. 2
Fig. 2
Survival analysis of 3PN IVF or ICSI compared to their respective 2PN controls. Fisher’s exact test was used to test the hypothesis of no difference between the 3 PN and 2 PN groups. Significant differences (p < 0.05) between 3 PN embryos and the 2 PN embryos are marked with a for the IVF embryos and b for the ICSI embryos, respectively
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References

    1. Grossmann M, Calafell JM, Brandy N, Vanrell JA, Rubio C, Pellicer A, et al. Origin of tripronucleate zygotes after intracytoplasmic sperm injection. Hum Reprod. 1997;12:2762–2765. doi: 10.1093/humrep/12.12.2762. - DOI - PubMed
    1. Staessen C, Van Steirteghem AC. The chromosomal constitution of embryos developing from abnormally fertilized oocytes after intracytoplasmic sperm injection and conventional in-vitro fertilization. Hum Reprod. 1997;12:321–327. doi: 10.1093/humrep/12.2.321. - DOI - PubMed
    1. Yu SL, Lee RK, Su JT, Chih YF, Tsai YC, Lin MH, et al. Distinction between paternal and maternal contributions to the tripronucleus in human zygotes obtained after in vitro fertilization. Taiwan J Obstet Gynecol. 2006;45:313–316. doi: 10.1016/S1028-4559(09)60249-7. - DOI - PubMed
    1. Jacobs PA, Szulman AE, Funkhouser J, Matsuura JS, Wilson CC. Human triploidy: relationship between parental origin of the additional haploid complement and development of partial hydatidiform mole. Ann Hum Genet. 1982;46:223–231. doi: 10.1111/j.1469-1809.1982.tb00714.x. - DOI - PubMed
    1. Jauniaux E, Brown R, Rodeck C, Nicolaides KH. Prenatal diagnosis of triploidy during the second trimester of pregnancy. Obstet Gynecol. 1996;88:983–989. doi: 10.1016/S0029-7844(96)00330-4. - DOI - PubMed

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