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. 1987 Oct;410(3):335-7.
doi: 10.1007/BF00580285.

A simple method of fast extracellular solution exchange for the study of whole-cell or single channel currents using patch-clamp technique

S Hering  1 , D J Beech, T B Bolton
Affiliations

A simple method of fast extracellular solution exchange for the study of whole-cell or single channel currents using patch-clamp technique

S Hering et al. Pflugers Arch. 1987 Oct.

Abstract

A new concentration-jump technique was devised for the rapid application of drugs to single, isolated cells attached to the base of the experimental chamber while recording from them with patch-clamp technique. Cells were placed in a micro-drop (less than 0.1 microliter) in a small inner bath which was separated from an outer bath by a ring of "Sylgard" polymer. Stable whole-cell recordings were made in the micro-drop and rapid solution exchange took place when a much larger volume of test solution from the outer bath was flooded over the Sylgard ring and mixed with the micro-drop. Complete equilibration occurred within less than 10 ms.

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