doi: 10.1007/BF00385105.
[Localisation of the α-amanitin-sensitive RNA-polymerase in nuclei of Acetabularia]
[Article in
German]
Affiliations
- PMID: 24469574
- DOI: 10.1007/BF00385105
Item in Clipboard
[Localisation of the α-amanitin-sensitive RNA-polymerase in nuclei of Acetabularia]
[Article in
German]
Planta.
1973 Sep.
Abstract
An isolation medium for nuclei of Acetabularia mediterranea is described. The medium offers the possibility to keep nuclei alive (verified by reimplantation) for at least 10 min and to obtain nuclei with radioactively labelled RNA.The influence of α-amanitin on nucleolar and nucleoplasmatic RNA-synthesis is investigated autoradiographically. Amanitin inhibits the incorporation of uridine into the nucleoplasmatic RNA, whereas incorporation into the RNA of nucleoli is hardly or not at all influenced.We conclude that, in agreement with findings on animal cells, α-amanitin inhibits at least one of the RNA-polymerases localized in the nucleoplasm but does not markedly influence the RNA-polymerase of the nucleoli.
Similar articles
-
Inhibition of hepatic deoxyribonucleic acid-dependent ribonucleic acid polymerases by the exotoxin of Bacillus thuringiensis in comparison with the effects of -amanitin and cordycepin.Biochem J. 1972 Aug;129(1):153-66. doi: 10.1042/bj1290153. Biochem J. 1972. PMID: 4539593 Free PMC article.
-
Effects of alpha-amanitin on RNA synthesis by mouse embryos in culture.J Exp Zool. 1978 Mar;203(3):351-60. doi: 10.1002/jez.1402030303. J Exp Zool. 1978. PMID: 641476
-
Role of DNA-dependent RNA polymerase 3 in the transcription of the tRNA and 5S RNA genes.Proc Natl Acad Sci U S A. 1974 May;71(5):1790-4. doi: 10.1073/pnas.71.5.1790. Proc Natl Acad Sci U S A. 1974. PMID: 4525293 Free PMC article.
-
Role of DNA-dependent RNA polymerases II and III in transcription of the adenovirus genome late in productive infection.Proc Natl Acad Sci U S A. 1974 Sep;71(9):3426-39. doi: 10.1073/pnas.71.9.3426. Proc Natl Acad Sci U S A. 1974. PMID: 4530313 Free PMC article.
-
Hormonal control of transcription in the rat uterus. Stimulation of deoxyribonucleic acid-dependent RNA polymerase III by estradiol.J Biol Chem. 1977 Feb 10;252(3):1092-8. J Biol Chem. 1977. PMID: 838697
Cited by
-
Viability of Acetabularia nucleus after 24 hours in an artificial medium.Protoplasma. 1975;85(1):115-8. doi: 10.1007/BF01567763. Protoplasma. 1975. PMID: 1178900 No abstract available.
-
RNA synthesis in isolated living and fixed nuclei of Acetabularia.Protoplasma. 1977;93(1):45-53. doi: 10.1007/BF01276281. Protoplasma. 1977. PMID: 335455 No abstract available.