Influence of squid liver powder on accumulation of cadmium in serum, kidney and liver of mice

Abstract

In this study, the effect of squid liver powder intake on accumulation of cadmium in mice was investigated. Subjects were divided into 4 groups including the control group (CON), squid liver powder group with lipids not removed (SLP100), and squid liver powder groups with lipids removed (LFSLP50 and LFSLP100). Feed intake and food efficiency ratio of squid liver powder groups was significantly higher than the CON. As a result of investigating cadmium content in hair, serum, liver, and kidney during intake of squid liver powder, all groups showed increase in cadmium accumulation through consistent, long-term intake. Especially, cadmium content in liver and kidney of LFSLP100 was significantly higher than the content of SLP100 and CON. As a result of pathological observation on liver and kidney tissues according to squid liver powder diet, LFSLP100 showed most serious pathological symptoms. In case of kidney tissues, degeneration was significantly more severe in LFSLP100 compared to other groups. Such results suggest that cadmium concentration in human body can be increased by ingestion of whole squid including internal organs and that tissues can be damaged by increased cadmium concentration. More specific and systematic studies are deemed necessary.

Keywords: Todarodes pacificus; cadmium accumulation; heavy metal; rats; squid liver.

Fig. 1.

Photomicroscope of mouse liver after feeding of squid liver addition feed. A, C, E, G: 4 weeks. B, D, F, H: 8 weeks. C: Some hepatocytes were much swelled and their nuclei were also hyper chromatic, and some sinusoidal spaces were distorted. D: Many hepatocytes (arrows, D2) near hepatic artery were sclerosed with hyper chromatic nuclei. E: The portal vein was dilated and filled with red blood cells, and adjacent hepatocytes were degenerative with sclerotic change. F: The hepatic cords (arrows, F2) around portal vein was severely distorted and damaged. G: The hepatocytes between portal veins and hepatic aunties were relatively well presence, but some hepatocytes were degenerative in high magnification veins. H: Many hepatocytes (arrows, H2) became sclerotic and degenerative, and the associated portal vein was dilated and congested with red blood cells.

Fig. 2.

Photomicroscope of mouse kidney after feeding of squid liver addition feed. A, C, E, G: 4 weeks. B, D, F, H: 8 weeks. B: glomerulus (arrows) and convoluted ducts (arrow heads). The histological structure of glomerulus and convoluted ducts were well preserved. C: The glomerulus was much shrinkaged and the convoluted ducts were dilated. D: There appeared hemorrhage between the glomerulus and ducts were greatly dilated. E: The glomerulus were greatly shrinkage and sclerosed, and the convoluted ducts were remarkably distorted. F: A glomerulus was almost destroyed by hyaline necrosis and the surrounding ductal structure was dilated and degenerative. G: A glomerulus was relatively well preserved, while another glomerulus was almost destroyed. And the convoluted ducts were usually dilated but relatively well preserved. H: A glomerulus was lonely degenerative by hyaline charge, and the convoluted ducts were greatly dilated with the detachment of ductal cell.