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. 2013 Jan;2(1):36-52.
doi: 10.5812/nephropathol.8989. Epub 2013 Jan 1.

Symmetric dimethyl arginine and N-acetyl-β-D-glucosaminidase lysozimuria of proximal renal tubules as a target for nephrotoxicity in patients with rheumatoid arthritis treated with disease modifying antirheumatic drugs

Dejan Spasovski  1 , Arif Latifi  2 , Nada Marina  1 , Jordan Calovski  1 , Irena Kafedziska  1 , Gjorgi Božinovski  1 , Snežana Percinkova  1 , Maja Slaninka-Micevska  3 , Trajan Balkanov  3 , Beti Dejanova  4 , Sonja Alabakovska  5 , Svetlana Krstevska-Balkanov  2 , Goce Spasovski  6 , Dejan Spasovski  7
Affiliations

Symmetric dimethyl arginine and N-acetyl-β-D-glucosaminidase lysozimuria of proximal renal tubules as a target for nephrotoxicity in patients with rheumatoid arthritis treated with disease modifying antirheumatic drugs

Dejan Spasovski et al. J Nephropathol. 2013 Jan.

Abstract

Background: The aim of this study was to determine the effect of initial therapy with some disease modifying antirheumatic drugs (DMARDs) (Methotrexate and Ketoprofen) on glomerular and tubular integrity in patients with Rheumatoid arthritis (RA).

Objectives: To determine whether there is a change in clinical and laboratory indicators of renal function in course of the follow up of treatment and whether that change correlates with the dynamics of the quantity of enzymes excreted in urine and reactants of the acute phase.

Materials and methods: Using colorimetric method for determination of NAG, samples of 70 participants were examined (35 RA patients treated with Ketoprofen only, 35 RA patients treated with combined use of Methotrexate and Ketoprofen). The follow up was 5 time-intervals in the course of 24 weeks.

Results: There was moderate correlation between NAG and microalbuminuria (r=0,34) in the group of patients treated with Ketoprofen only, while statistically significant correlation (r=0,21) was seen in group of patients with combined use of Methotrexate and Ketoprofen. NAG enzymuria in size, number of patients registered, and time of appearance were greater and appears earlier in the group with the combined use of Methotrexate and Ketoprofen compared with the mono-therapy with Ketoprofen. Mean urinary NAG induction was increasing with the concomitant use of Methotrexate and Ketoprofen.

Conclusions: Methotrexate is more potent NAG inductor than Ketoprofen and provokes greater tubular enzymuria than Ketoprofen.

Keywords: Immunosuppressive drugs; N-acetyl-D-glucosaminidase; Rheumatoid arthritis; Symmetric dimethyl arginine.

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References

    1. Mellman I, Fuchs R, Helenius A. Acidification of the endocytic and exocytic pathways. Annu Rev Biochem . 1986;55:663–700. - PubMed
    1. Wellwood JM, Ellis BG, Price RG, Hammond K, Thompson AE, Jones NF. Urinary N-acetyl-beta-D-glucosaminidase activities in patients with renal disease. Br Med J . 1975;3:408–11. - PMC - PubMed
    1. Dos Santos AC, Colacciopo S, Dal Bo CM. Occupational exposure to lead, kidney function tests and blood pressure. Am J Int Med . 1994;26:635–43. - PubMed
    1. Shibasak T, Nakano H, Ohno I. Effect of pentoxifylline on CdCl -induced nephrotoxici-2ty in the rat. Biol Trace Elem Res . 1994;41:245–51. - PubMed
    1. Hong CY, Chia KS. Markers of diabetic nephropathy. J Diabetes Complications . 1998;12:43–60. - PubMed

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