Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Dec 23;15(24):10507-21.
doi: 10.1093/nar/15.24.10507.

Rate of degradation of [alpha]- and [beta]-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies

C Cazenave  1 , M Chevrier, T T Nguyen, C Hélène
Affiliations
Free PMC article

Rate of degradation of [alpha]- and [beta]-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies

C Cazenave et al. Nucleic Acids Res. .
Free PMC article

Abstract

End-labelled oligodeoxynucleotides were injected into Xenopus laevis oocytes and their degradation products were analysed by high-performance ion-exchange liquid chromatography after various times of incubation. The oligonucleotides were synthesised with either the natural [beta] anomers or the synthetic [alpha] anomers of deoxynucleotide units. Oligo-[beta] deoxynucleotides are short-lived inside oocytes (half-life approximately equal to 10 min). Covalent attachment of an intercalating agent to the 3'-phosphate and of a methylthiophosphate group at the 5'-end protects oligodeoxynucleotides against 3'- and 5'-exonucleases, respectively. The half-life of such substituted oligodeoxynucleotides is increased to 40 minutes. Oligo-[alpha]-deoxynucleotides are quite resistant to both endo and exonucleases inside Xenopus oocytes. After 8 hours only 40% of a 16-mer oligo-[alpha]-deoxynucleotide were hydrolysed. The rapid degradation of oligo-[beta]-deoxynucleotides suggests that efficient inhibition of translation in Xenopus oocytes involves an RNase H-induced hydrolysis of mRNAs hybridized to oligo-[beta]-deoxynucleotides.

PubMed Disclaimer

References

    1. Prog Nucleic Acid Res Mol Biol. 1985;32:291-320 - PubMed
    1. Methods Enzymol. 1983;101:370-86 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Jan;82(1):144-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5129-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jan;75(1):285-8 - PubMed