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. 1972 Dec;104(4):357-61.
doi: 10.1007/BF00386318.

The use of nurse culture in the development of haploid clones in tomato

W R Sharp  1 , R S Raskin, H E Sommer
Affiliations

The use of nurse culture in the development of haploid clones in tomato

W R Sharp et al. Planta. 1972 Dec.

Abstract

A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains. The grains are plated on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium. The plating efficiencies of such pollen cells ranges between 0 and 60%. The variability in the plating efficiencies and the potential use of this technique over that of anther culture are discussed.

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References

    1. Science. 1969 Nov 21;166(3908):1068-70 - PubMed
    1. Science. 1969 Jan 3;163(3862):85-7 - PubMed

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