Fine structure of abscission zones : IV. Effect of ethylene on the ultrastructure of abscission cells of tobacco flower pedicels

Abstract

The effect of ethylene on abscission of flower pedicels of tobacco plants has been investigated. For the first 2 h of exposure to C2H4, the pedicels bend rather than break in response to applied force, but after 2.5 h exposure they break at the abscission zone under an applied force of 40 g. The break strength of the abscission zone decreases exponentially with time to 5 g at 5 h after beginning of the C2H4 treatment. An examination of the tissue at the fine structural level 2 h after exposure to C2H4 reveals the accumulation of rough endoplasmic reticulum (RER) in the abscission cells. Rough ER becomes increasingly abundant by 3-5 h exposure of the tissue to C2H4. There is approximately a 30 fold increase in RER by 5 h of exposure, as compared to untreated tissue.Loss in the integrity of the membranes of microbodies occurs by 5 h exposure of the tissue to C2H4. As cell wall degradation proceeds, fibrous material, vesicular structures, and electron dense bodies-the latter often appearing striated-develop in the disintegrating wall. Little change is seen in the structure of nuclei, mitochondria, chloroplasts and in the crystalloid cores of microbodies during the first 5 h of exposure of the tissue to C2H4. However, disorganization of cytoplasmic components does occur in cells where cell wall breakdown is at an advanced stage.