Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Aug 8;23(1):129-133.
doi: 10.1007/s00580-012-1583-6. eCollection 2014.

Isolation and identification of Vibrio parahaemolyticus from seawater and sediment samples in the southern coast of the Caspian Sea

Majid Alipour  1 , Khosro Issazadeh  2 , Javad Soleimani  2
Affiliations

Isolation and identification of Vibrio parahaemolyticus from seawater and sediment samples in the southern coast of the Caspian Sea

Majid Alipour et al. Comp Clin Path. .

Abstract

The objectives of this study were to investigate the occurrence of Vibrio parahaemolyticus in the seawater and its sediment by molecular techniques and conventional microbiological methods. Of 300 samples analyzed, 20.3 % was recorded positive for V. parahaemolyticus. Of the 62 strains isolated, 26 (8.3 %) were obtained from the seawater samples, and 36 (12 %); from sediments. Only three strains (4.83 %) showed hemolytic activity in Wagatsuma agar. The results of this study demonstrated the presence of V. parahaemolyticus in the southern coast of the Caspian Sea (Northern Iran). Furthermore, the PCR approach proved useful for reliable confirmation of species identification. V. parahaemolyticus is an important human pathogen responsible for food-borne gastroenteritis worldwide. These findings indicated the potential sanitary risk associated with the presence of pathogenic V. parahaemolyticus in the Caspian Sea.

Keywords: Acute gastroenteritis; Seawater; Sediment; Vibrio parahaemolyticus.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Area of study and locations of the sampling sites in the southern coast of the Caspian Sea (Northern Iran). Site 1 Bandar Torkaman, site 2 Sari, site 3 Noor, site 4 Chalus, site 5 Ramsar, site 6 Chamkhaleh, site 7 Bandar Anzali
See this image and copyright information in PMC

References

    1. Alam MJ, Tomochika KI, Miyoshi SI, et al. Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan. FEMS Microbiol Lett. 2002;208:83–87. doi: 10.1111/j.1574-6968.2002.tb11064.x. - DOI - PubMed
    1. Bisha B, Simonson J, Janes M, et al. A review of the current status of cultural and rapid detection of Vibrio parahaemolyticus. Int J Food Sci Technol. 2012;47:885–899. doi: 10.1111/j.1365-2621.2012.02950.x. - DOI
    1. Broberg CA, Calder TJ, Orth K. Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes Infect. 2011;13:992–1001. doi: 10.1016/j.micinf.2011.06.013. - DOI - PMC - PubMed
    1. Cabrera-Garcia ME, Vazquez-Salinas C, Quinones-Ramirez EI, et al. Serologic and molecular characterization of Vibrio parahaemolyticus strains isolated from seawater and fish products of the Gulf of Mexico. Appl Environ Microbiol. 2004;70:6401–6406. doi: 10.1128/AEM.70.11.6401-6406.2004. - DOI - PMC - PubMed
    1. Canizalez-Roman A, Flores-Villasenor H, Zazueta-Beltran J, et al. Comparative evaluation of a chromogenic agar medium–PCR protocol with a conventional method for isolation of Vibrio parahaemolyticus strains from environmental and clinical samples. Can J Microbiol. 2011;57:136–142. doi: 10.1139/W10-108. - DOI - PubMed

LinkOut - more resources