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. 2014;28(10):760-3.
doi: 10.1080/14786419.2013.879304. Epub 2014 Jan 31.

Activity-guided isolation of antioxidants from the leaves of Terminalia arjuna

Pradeep Pratap Singh  1 , S M S Chauhan
Affiliations

Activity-guided isolation of antioxidants from the leaves of Terminalia arjuna

Pradeep Pratap Singh et al. Nat Prod Res. 2014.

Abstract

An activity-guided isolation and purification process was used to identify the l,l-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging components of the food plant (Terminalia arjuna) of Antheraea mylitta. Dry leaves of T. arjuna were extracted with different solvents and tested for their antioxidant activity against DPPH(•). The acetone-water (8:2) extract expressed strong DPPH radical-scavenging activity, and was subjected to column chromatography over silica gel. Gallic acid, apigenin, luteolin, quercetin, epicatechin, ellagic acid and 1-O-β-galloyl glucose were isolated as active components and characterised by using different spectroscopic techniques.

Keywords: Antheraea mylitta; DPPH•; antioxidant; cardioprotective.

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