Avian scale development: XI. Immunoelectron microscopic localization of alpha and beta keratins in the scutate scale

Abstract

Epithelial-mesenchymal interactions play important roles in morphogenesis, histogenesis, and keratinization of the vertebrate integument. In the anterior metatarsal region of the chicken, morphogenesis results in the formation of distinct overlapping scutate scales. Recent studies have shown that the dermis of scutate scales is involved in the expression of the beta keratin gene products, which characterize terminal differentiation of the epidermis on the outer scale surface (Sawyer et al.: Dev. Biol. 101:8-18, '84; Shames and Sawyer: Dev. Biol. 116:15-22, '86; Shames and Sawyer: In A.A. Moscona and A. Monroy (eds), R.H. Sawyer (Vol. ed): Current Topics in Developmental Biology. Vol. 22: The Molecular and Developmental Biology of Keratins. New York: Academic Press, pp. 235-253, '87). Since alpha and beta keratins are both found in the scutate scale and are members of two different multigene families, it is important to know the precise location of these distinct keratins within the epidermis. In the present study, we have used protein A-gold immunoelectron microscopy with antisera made against avian alpha and beta keratins to specifically localize these keratins during development of the scutate scale to better understand the relationship between dermal cues and terminal differentiation. We find that the bundles of 3-nm filaments, characteristic of tissues known to produce beta keratins, react specifically with antiserum which recognizes beta keratin polypeptides and are found in the embryonic subperiderm that covers the entire scutate scale and in the stratum intermedium and stratum corneum making up the platelike beta stratum of the outer scale surface. Secondly, we find that 8-10-nm tonofilaments react specifically with antiserum that recognizes alpha keratin polypeptides and are located in the germinative basal cells and the lowermost cells of the stratum intermedium of the outer scale surface, as well as in the embryonic alpha stratum, which is lost from the outer surface of the scale at hatching. The alpha keratins are found throughout the epidermis of the inner surface of the scale and the hinge region. Thus, the present study further supports the hypothesis that the tissue interactions responsible for the formation of the beta stratum of scutate scales do not directly activate the synthesis of beta keratins in the germinative cells but influence these cells so that they or their progeny will activate specific beta keratin genes at the appropriate time and place.