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. 2014 Apr:86:52-6.
doi: 10.1016/j.apradiso.2013.12.036. Epub 2014 Jan 8.

⁹⁹mTc carbonyl DTPA-rituximab: preparation and preliminary bioevaluation

Usha Pandey  1 , Mythili Kameswaran  1 , Haladhar Dev Sarma  2 , Grace Samuel  3
Affiliations

⁹⁹mTc carbonyl DTPA-rituximab: preparation and preliminary bioevaluation

Usha Pandey et al. Appl Radiat Isot. 2014 Apr.

Abstract

The anti CD20 antibody Rituximab was conjugated with para isothiocyanato benzyl diethylene triamine penta acetic acid (p-NCS-Bz-DTPA) and subsequent radiolabeling with (99m)Tc was carried out via the (99m)Tc carbonyl synthon. The (99m)Tc labeled antibody conjugate exhibited >95% radiochemical purity after purification and retained good in vitro stability when studied up to 24h at room temperature. In vitro cell binding studies carried out in Raji cells expressing CD20 antigen validated the biological efficacy of the preparation.

Keywords: (99m)Tc carbonyl–DTPA–Rituximab; Non-Hodgkin's lymphoma (NHL); Radioimmunoscintigraphy; Rituximab.

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