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. 2014 Feb 28;445(1):54-7.
doi: 10.1016/j.bbrc.2014.01.106. Epub 2014 Jan 31.

Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism

Jeeraporn Kansup  1 , Daisuke Tsugama  2 , Shenkui Liu  3 , Tetsuo Takano  4
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Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism

Jeeraporn Kansup et al. Biochem Biophys Res Commun. .

Abstract

Heterotrimeric G proteins (Gα, Gβ and Gγ) have pleiotropic roles in plants, but molecular mechanisms underlying them remain to be elucidated. Here we show that Arabidopsis Gβ (AGB1) interacts with NPH3, a regulator of phototropism. Yeast two-hybrid assays, in vitro pull-down assays and bimolecular fluorescence complementation assays showed that AGB1 and NPH3 physically interact. NPH3-null mutation (nph3) is known to completely abolish hypocotyl phototropism. Loss-of-function mutants of AGB1 (agb1-1 and agb1-2) showed decreased hypocotyl phototropism, and agb1/nph3 double mutants showed no hypocotyl phototropism. These results suggest that AGB1 is involved in the NPH3-mediated regulation of phototropism.

Keywords: Arabidopsis; Heterotrimeric G protein β subunit; Nonphototropic hypocotyl 3; Phototropism; Protein–protein interaction.

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