Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Mar;99(1):12-20.
doi: 10.1007/BF00392116.

Plating of isolated tobacco mesophyll protoplasts on agar medium

T Nagata  1 , I Takebe
Affiliations

Plating of isolated tobacco mesophyll protoplasts on agar medium

T Nagata et al. Planta. 1971 Mar.

Abstract

A technique was developed to derive cell and plant clones from isolated mesophyll protoplasts of tobacco. The protoplasts, plated on a fully defined agar medium, divided and grew actively forming visible colonies after one month of culture. Efficiency of colony formation depended on cell density and light condition during incubation. Under standard conditions, 60% of plated protoplasts formed colonies. Upon transfer onto suitable media, these colonies differentiated shoots and roots, and eventually regenerated whole plants. Advantages of mesophyll protoplasts as the source of clones as well as implication of the plating technique for genetical studies are discussed.

PubMed Disclaimer

References

    1. Dev Growth Differ. 1969 Oct;11(2):143-51 - PubMed
    1. Exp Cell Res. 1965 Oct;40(1):85-95 - PubMed
    1. J Gen Physiol. 1960 Mar;43:841-51 - PubMed
    1. Plant Physiol. 1965 May;40(3):520-8 - PubMed
    1. Science. 1970 Apr 24;168(3930):487-9 - PubMed

LinkOut - more resources