Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Feb;51(2):341-6.
doi: 10.1007/s13197-011-0498-6. Epub 2011 Aug 20.

Biological activities of fructooligosaccharide (FOS)-containing Coix lachryma-jobi Linn. extract

Jiradej Manosroi  1 , Narinthorn Khositsuntiwong  2 , Aranya Manosroi  1
Affiliations

Biological activities of fructooligosaccharide (FOS)-containing Coix lachryma-jobi Linn. extract

Jiradej Manosroi et al. J Food Sci Technol. 2014 Feb.

Abstract

Fructooligosaccharide (FOS), a prebiotic was extracted from the grain of Coix lachryma-jobi Linn. (Job's tears) by hot water extraction at 60 °C for 1 h. The resulting dried powder extract was assayed for FOS content of 1-kestose (GF2), nystose (GF3) and 1-β-D-fructofuranosylnystose (GF4) using HPLC equipped with RI detector. Total FOS content of the extract was 24.98 ± 7.48% (g/100 g crude extract). The biological activity including antioxidant and cytotoxicity of the FOS-containing extract was determined. The antioxidant activity by DPPH free radical scavenging of FOS-containing extract was comparable to vitamin C (0.97 fold of vitamin C) with a slight lipid peroxidation inhibition activity. The extract exhibited no cytotoxic effect on normal human skin fibroblast. These results have confirmed not only the source of FOS from Job's tears extract but also its potential application as antioxidant in food or cosmetic products.

Keywords: Antioxidant; Coix lachryma-jobi Linn.; Fructooligosaccharide; Job’s tears.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Chromatogram of FOS from Coix lachryma-jobi Linn. (Job’s tears) grain extract (GF2, GF3 and GF4 indicated 1-kestose, nystose and 1-β-D-fructofuranosylnystose, respectively)
Fig. 2
Fig. 2
Percentages of cell growth (% G) of the normal human skin fibroblast treated with standard vitamin C, standard FOS and FOS-containing Coix lachryma-jobi Linn. (Job’s tears) extract determined by SRB assay (n = 3)
See this image and copyright information in PMC

References

    1. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. J Food Sci Technol. 1995;28(1):25–30.
    1. Bunce TJ, Howard MD, Allee GL, Pace LW. Protective effects of fructooligosaccharide (FOS) in prevention of mortality and morbidity from infectious E. coli K:88 challenge. J Anim Sci. 1995;73(Suppl. 1):69. - PubMed
    1. Choi KH, Namhrng H, Paid IK. Effects of dietary fructo-oligosaccharide on the suppression of intestinal colonization of Salmonella typhimurium in broiler chickens. Korean J Anim Sci. 1994;36:271–284.
    1. Dechsakulthorn F, Hayes A, Bakand S, Joeng L, Winder C (2007) In vitro cytotoxicity assessment of selected nanoparticles using human skin fibroblasts. Proc 6th World Congress on Alternatives & Animal Use in the Life Sciences 14:21–25
    1. Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food. J Agr Food Chem. 1990;38:674–677. doi: 10.1021/jf00093a019. - DOI

LinkOut - more resources