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. 1987;88(1):61-4.
doi: 10.1007/BF00490169.

Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies

M Geffard  1 , S Tuffet, N Mons, J L Chagnaud
Affiliations

Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies

M Geffard et al. Histochemistry. 1987.

Abstract

Using a monoclonal antibody against dopamine and a rabbit antiserum against serotonin, 5-methoxytryptamine or tryptamine, we were able to achieve the simultaneous localization of two amines in glutaraldehyde-fixed sections of rat dorsal raphe nuclei. In this staining procedure, the first antigen was localized using 3,3'-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphthol basic dye (2-NBD) method. The two antigens were localized in different cells or structures. No overlap of the staining was observed, thus indicating that dopamine is not localized with serotonin, 5-methoxytryptamine or tryptamine.

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