Analysis of the incompatibility determinants of I-complex plasmids
- PMID: 2449421
- PMCID: PMC210908
- DOI: 10.1128/jb.170.3.1311-1318.1988
Analysis of the incompatibility determinants of I-complex plasmids
Abstract
The isolation and characterization of minireplicons corresponding to group B and I-complex plasmids are reported. The molecular structures of the small RNAs that may play a major role in the replication control and incompatibility reactions of the plasmids are compared. A mutant plasmid with changed copy number and incompatibility specificity is described. This mutant had a single-base-pair substitution in the DNA region that codes for the small RNA.
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