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. 2013 Oct;8(10):doi: 10.4161/psb.26758.
doi: 10.4161/psb.26758.

Analysis of UV-B regulated miRNAs and their targets in maize leaves

Paula Casati

Analysis of UV-B regulated miRNAs and their targets in maize leaves

Paula Casati. Plant Signal Behav. 2013 Oct.

Abstract

UV-B radiation elicits physiological and developmental changes in plants. In this work, to characterize the mechanisms of gene regulation by UV-B radiation, the global miRNA expression profiles in juvenile maize leaves were compared under control conditions in the absence of UV-B and after an 8 h-UV-B treatment similar to ambient UV-B levels using small RNA microarrays. Seventeen miRNA s were identified that were differentially expressed in response to UV-B. In parallel, the expression of putative mRNA targets was examined in mRNA microarrays using the same RNA samples used for the small RNA analysis. In general, there is a strong negative correlation to the miRNA expression. Thus, post-transcriptional regulation is an important step in gene expression by UV-B radiation in maize.

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Figures

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Figure 1. Expression of UV-B regulated miRNAs and their targets. (A) Analysis of microarray data of UV-B regulated miRNAs, the numbers correspond to log2 ratios of UV-B/C leaf samples. (B-H) Comparative expression patterns of a subset of UV-B regulated miRNAs and their (putative) targets obtained from miRNA and mRNA microarray analysis, respectively, using the same total RNA sample. The numbers correspond to log2 ratios of UV-B/C leaf samples.
See this image and copyright information in PMC

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