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. 1987 Sep;7(3):317-22.
doi: 10.1007/BF00711308.

Maitotoxin stimulates phosphoinositide breakdown in neuroblastoma hybrid NCB-20 cells

F Gusovsky  1 , T Yasumoto, J W Daly
Affiliations

Maitotoxin stimulates phosphoinositide breakdown in neuroblastoma hybrid NCB-20 cells

F Gusovsky et al. Cell Mol Neurobiol. 1987 Sep.

Abstract

1. Maitotoxin (MTX) was an extraordinarily potent stimulant of phosphoinositide breakdown in the neuroblastoma hybrid NCB-20 cells. 2. Maximal responses were obtained at 0.25-0.5 ng MTX/ml, and resulted in increased formation of [3H]inositol mono-, bis-, and trisphosphates. Increased formation of [3H]inositol bis- and trisphosphate was observed as early as 15 sec after the addition of MTX. 3. MTX-induced phosphoinositide breakdown in NCB-20 cells was not antagonized by organic (nifedipine, methoxyverapamil) or inorganic (Mn2+, Co2+, Cd2+) calcium channel blockers. However, the response on phosphoinositide breakdown was completely eliminated in the absence of extracellular calcium. 4. The results suggest that MTX either directly stimulates phosphoinositide breakdown in a calcium-dependent manner or acts indirectly through calcium channels insensitive to organic/inorganic calcium channel blockers.

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References

    1. Berridge, M. J., Dawson, R. M. C., Downes, C. P., Heslop, J. P., and Irvine, R. F. (1983). Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.Biochem. J.212473–482. - PMC - PubMed
    1. Berta, P., Sladeczek, F., Derancourt, J., Durand, M., Travo, P., and Haiech, J. (1986). Maitotoxin stimulates the formation of inositol phosphates in rat aortic myocytes.FEBS Lett.197349–352. - PubMed
    1. Chuang, D. (1986). Carbachol-induced accumulation of inositol-1-phosphate in neurohybridoma NCB-20 cells: Effects of lithium and phorbol esters.Biochem. Biophys. Res. Comm.136622–629. - PubMed
    1. Freedman, S. B., Miller, R. J., Miller, D. M., and Tindall, D. R. (1984). Interactions of maitotoxin with voltage-sensitive calcium channels in cultured neuronal cells.Proc. Natl. Acad. Sci. USA814582–5858. - PMC - PubMed
    1. Kobayashi, M., Ohizumi, Y., and Yasumoto, T. (1985). The mechanism of action of maitotoxin in relation to Ca2+ movements in guinea pig and rat cardiac muscles.Br. J. Pharmacol.86385–391. - PMC - PubMed

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