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. 2014 Feb 6:4:3999.
doi: 10.1038/srep03999.

Evaluation of pleural effusion sCD26 and DPP-IV as diagnostic biomarkers in lung disease

Nuria Sánchez-Otero  1 , Francisco Javier Rodríguez-Berrocal  1 , María Páez de la Cadena  1 , María Isabel Botana-Rial  2 , Oscar J Cordero  3
Affiliations

Evaluation of pleural effusion sCD26 and DPP-IV as diagnostic biomarkers in lung disease

Nuria Sánchez-Otero et al. Sci Rep. .

Abstract

In this study, we measured ADA and DPP-IV enzymatic activity and sCD26 concentration in 150 pleural effusion (PE) samples and tested for correlations between these and other cellular and biochemical measures. We found that DPP-IV in particular might improve the specificity (but not the sensitivity) of the ADA test for diagnosis of pulmonary tuberculosis, since half of the false ADA positive results in non-tuberculous PE were also DPP-IV positive. A percentage of patients with malignant PE were sCD26 or DPP-IV positive; however, some patients with benign PE also tested positive. As a pattern associated with DPP-IV (but not the CD26 protein) was observed in PE, we searched for a finding that might increase the value of these biomarkers for diagnosis of malignancy. The observed pattern was related to the presence of leukocytes, as indicated by correlations with the cell count, and to a band of 180 kDa, detected by immunoblotting.

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Figures

Figure 1
Figure 1
Levels of (A) ADA enzymatic activity, (B) DPP-IV enzymatic activity and (C) sCD26 concentration in pleural effusion (PE) samples from patients diagnosed with the following types of PE, from left to right: benign PE (circles), including tuberculous, parapneumonic, paramalignant, non neoplasic and miscellaneous PE; malignant PE (triangles), including epithelial, mesothelioma and lymphoma PE.
Figure 2
Figure 2
Correlations between (A) sCD26 concentration and DPP-IV activity, and (B) ADA activity and sCD26 concentration, in some types of PE. Spearman's correlation coefficient (R) and p values are shown on the graphs; (C) Correlation between these biomarkers and the MC counts in BPE or MPE groups.
Figure 3
Figure 3
(A) Western blot of PE sCD26 with anti-CD26 mAb TP1/16 according to the sample conditions used in SDS-PAGE.(B) The same western blot in samples from different pathologies showing the band of 180 kDa (arrows) mainly in MPE, which was absent or weaker in BPE samples. No clear trend was observed for the other bands of lower MW. Gel analyses were repeated 3–4 times.
See this image and copyright information in PMC

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