Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Apr;62(4):1132-5.
doi: 10.1128/JVI.62.4.1132-1135.1988.

A fast and sensitive method for detecting specific viral RNA in mammalian cells

U Paeratakul  1 , P R De Stasio, M W Taylor
Affiliations

A fast and sensitive method for detecting specific viral RNA in mammalian cells

U Paeratakul et al. J Virol. 1988 Apr.

Abstract

A quick and sensitive method to quantitate viral RNA synthesis has been developed. Utilizing glutaraldehyde to fix infected cells onto nitrocellulose paper, viral RNA can be probed directly in situ. Viral message can be detected from as few as 10(4) infected cells. This technique can be used to study viral gene expression and can be adapted to screen the activity of antiviral agents such as interferon.

PubMed Disclaimer

References

    1. Cell. 1981 May;24(2):385-91 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1458-62 - PubMed
    1. Nucleic Acids Res. 1986 Oct 10;14(19):7597-615 - PubMed
    1. DNA. 1983;2(3):243-54 - PubMed
    1. Nucleic Acids Res. 1984 Mar 26;12(6):2969-85 - PubMed

Publication types

LinkOut - more resources