Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Mar;24(3):230-8.
doi: 10.1007/BF02623552.

Attachment and multiplication, morphology and protein production of human fetal primary liver cells cultured in hormonally defined media

M Salas-Prato  1 , J F Tanguay, Y Lefebvre, D Wojciechowicz, H H Liem, D W Barnes, G Ouellette, U Muller-Eberhard
Affiliations

Attachment and multiplication, morphology and protein production of human fetal primary liver cells cultured in hormonally defined media

M Salas-Prato et al. In Vitro Cell Dev Biol. 1988 Mar.

Erratum in

  • In Vitro Cell Dev Biol 1988 May;24(5):470

Abstract

We established for human fetal liver cells (cultured for 2 wk) in a hormonally defined medium, optimal conditions for attachment, multiplication, and preservation of epithelial morphology as well as production and secretion of serum proteins characteristic of fetal (alpha l-fetoprotein, AFP) and adult (albumin and hemopexin) life. Conditions were considered optimal when cell number, albumin, and hemopexin levels were maintained throughout the 2-wk culture period. However, the decrease in AFP concentration, which occurred after a few days of culture, could not be reversed. The culture system developed is a suitable model for studying regulatory mechanisms governing structure and function during differentiation and may prove useful for testing the effect of toxic agents during fetal development of the human liver.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1984 Jan 13;118(1):168-75 - PubMed
    1. Biochem Pharmacol. 1982 Jul 15;31(14):2427-30 - PubMed
    1. Cell Tissue Res. 1981;218(1):13-21 - PubMed
    1. Eur J Biochem. 1985 Jan 2;146(1):155-9 - PubMed
    1. Oncodev Biol Med. 1982;3(5-6):343-50 - PubMed

Publication types

LinkOut - more resources