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. 2014 Jan 2:67:2.22.1-2.22.36.
doi: 10.1002/0471142956.cy0222s67.

How to build a time-gated luminescence microscope

Dayong Jin  1 , Yiqing Lu  1 , Robert C Leif  2 , Sean Yang  2 , Megha Rajendran  3 , Lawrence W Miller  3
Affiliations

How to build a time-gated luminescence microscope

Dayong Jin et al. Curr Protoc Cytom. .

Abstract

The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.

Keywords: autofluorescence; lanthanide; microscopy; time-gated luminescence.

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References

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