Characterization of the Asian Citrus Psyllid Transcriptome

Justin Reese¹, Matthew K Christenson², Nan Leng³, Surya Saha⁴, Brandi Cantarel¹, Magdalen Lindeberg⁴, Cecilia Tamborindeguy⁵, Justin Maccarthy¹, Daniel Weaver¹, Andrew J Trease², Ready Steven V², Vincent M Davis⁶, Courtney McCormick³, Christian Haudenschild³, Shunsheng Han⁷, Shannon L Johnson⁷, Kent S Shelby⁸, Hong Huang⁹, Blake R Bextine¹⁰, Robert G Shatters¹¹, David G Hall¹¹, Paul H Davis¹², Wayne B Hunter¹¹

Affiliations

¹ Genformatic, LLC, 6301 Highland Hills Drive Austin, TX 78731, USA.
² Department of Biology, University of Nebraska at Omaha, Omaha, Nebraska 68182, USA.
³ Illumina, Inc., 25861 Industrial Blvd, Hayward, California 94545, USA.
⁴ Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca New York 14853, USA.
⁵ Department of Entomology, Texas A&M University, 2475 TAMU College Station, Texas 77843, USA.
⁶ Heteroskedastic, Inc., Arvada, Colorado, USA.
⁷ Los Alamos National Laboratory Bioscience division B-6, MS M888 Los Alamos, New Mexico 87545, USA.
⁸ USDA Agricultural Research Service, 1503 South Providence Road, Columbia, Missouri 65203, USA.
⁹ School of Information, University South Florida, 4202 East Fowler Avenue, Tampa, FL 33260, USA.
¹⁰ University of Texas at Tyler, 3900 University Boulevard, Tyler, TX, 75799, USA.
¹¹ USDA Agricultural Research Service, 2001 South Rock Road, Fort Pierce, FL 34945, USA.
¹² Department of Biology, University of Nebraska at Omaha, Omaha, Nebraska 68182, USA. ; Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska 68182, USA.

PMID: 24511328
PMCID: PMC3914308
DOI: 10.7150/jgen.7692

Characterization of the Asian Citrus Psyllid Transcriptome

Justin Reese et al. J Genomics. 2014.

. 2014 Jan 1:2:54-58.

doi: 10.7150/jgen.7692.

Authors

Affiliations

¹ Genformatic, LLC, 6301 Highland Hills Drive Austin, TX 78731, USA.
² Department of Biology, University of Nebraska at Omaha, Omaha, Nebraska 68182, USA.
³ Illumina, Inc., 25861 Industrial Blvd, Hayward, California 94545, USA.
⁴ Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca New York 14853, USA.
⁵ Department of Entomology, Texas A&M University, 2475 TAMU College Station, Texas 77843, USA.
⁶ Heteroskedastic, Inc., Arvada, Colorado, USA.
⁷ Los Alamos National Laboratory Bioscience division B-6, MS M888 Los Alamos, New Mexico 87545, USA.
⁸ USDA Agricultural Research Service, 1503 South Providence Road, Columbia, Missouri 65203, USA.
⁹ School of Information, University South Florida, 4202 East Fowler Avenue, Tampa, FL 33260, USA.
¹⁰ University of Texas at Tyler, 3900 University Boulevard, Tyler, TX, 75799, USA.
¹¹ USDA Agricultural Research Service, 2001 South Rock Road, Fort Pierce, FL 34945, USA.
¹² Department of Biology, University of Nebraska at Omaha, Omaha, Nebraska 68182, USA. ; Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska 68182, USA.

PMID: 24511328
PMCID: PMC3914308
DOI: 10.7150/jgen.7692

Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a vector for the causative agents of Huanglongbing, which threatens citrus production worldwide. This study reports and discusses the first D. citri transcriptomes, encompassing the three main life stages of D. citri, egg, nymph and adult. The transcriptomes were annotated using Gene Ontology (GO) and insecticide-related genes within each life stage were identified to aid the development of future D. citri insecticides. Transcriptome assemblies and other sequence data are available for download at the International Asian Citrus Psyllid Genome Consortium website [http://psyllid.org/download] and at NCBI [http://www.ncbi.nlm.nih.gov/bioproject/29447].

Keywords: Asian Citrus Psyllid; Diaphorina citri Kuwayama.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

**Figure 1**
Gene Ontology (GO) analysis of the representative transcripts present within the three life stages of *Diaphorina citri*. GO-terms were assigned to the representative transcripts of the three *D. citri* life stages and *Acyrthosiphon pisum*, *Tribolium castaneum*, *Pediculus humanus* and *Nasonia vitripennis*, the four organisms that exhibited the greatest number of top BLAST hits, using B2G4Pipe and then sorted into groups within three independent compartments using Blast2GO. The 'Cellular Process', 'Binding' and 'Organelle' groups contain the greatest number of representative transcripts within the 'Biological Processes', 'Molecular Function' and 'Cellular Component' domains, respectively. The representative transcriptomes of the four organisms and the egg, nymph and adult stages exhibited similar patterns, and thus indicates the completeness of the stage transcriptomes.

**Figure 2**
Genes related to insecticide targets, detoxification, and resistance within each of the three life stages of *Diaphorina citri*. Identification of putative *D. citri* insecticide-related genes was accomplished by comparing the representative transcripts from the three *D. citri* life stages to a custom protein database containing homologous genes of interest from closely related organisms using BLASTX. (A) *D. citri* predicted gene products of putative insecticide targets and predicted gene products putatively involved in detoxification and resistance were identified in all three life stages. (B) *D. citri* homologs of the major gene products involved in insect juvenile hormone metabolism are present within each life stage.

See this image and copyright information in PMC

References

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Characterization of the Asian Citrus Psyllid Transcriptome

Affiliations

Characterization of the Asian Citrus Psyllid Transcriptome

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

References

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