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. 1988 Apr;89(4):511-2.
doi: 10.1093/ajcp/89.4.511.

Iron pyronine and alcian blue for staining acid mucin in plastic-embedded sections

M S Cannon  1 , N R Stuth, J J Darcy
Affiliations

Iron pyronine and alcian blue for staining acid mucin in plastic-embedded sections

M S Cannon et al. Am J Clin Pathol. 1988 Apr.

Abstract

Iron pyronine Y and Alcian blue demonstrated sulfated and nonsulfated acid mucin, respectively, in plastic-embedded sections. Safran used in combination with these two dyes stained collagen and some reticulum fibers. Sulfated acid mucin was red, while nonsulfated acid mucin stained blue; collagen appeared yellow to greenish yellow. The iron pyronine Y-Alcian blue-safran staining method, when used as in the present article, produces excellent cellular visualization of sulfated and nonsulfated acid mucin.

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