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. 2014 Apr;90(4):690-6.
doi: 10.4269/ajtmh.13-0131. Epub 2014 Feb 10.

An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant protein Pap31 for detection of antibody against Bartonella bacilliformis infection among the Peruvian population

Nasikarn Angkasekwinai  1 , Erin H Atkins, Sofia Romero, John Grieco, Chien Chung Chao, Wei Mei Ching
Affiliations

An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant protein Pap31 for detection of antibody against Bartonella bacilliformis infection among the Peruvian population

Nasikarn Angkasekwinai et al. Am J Trop Med Hyg. 2014 Apr.

Abstract

Reliable laboratory testing is of great importance to detect Bartonella bacilliformis infection. We evaluated the sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA) using recombinant protein Pap31 (rPap31) for the detection of antibodies against B. bacilliformis as compared with immunofluorescent assay (IFA). Of the 302 sera collected between 1997 and 2000 among an at-risk Peruvian population, 103 and 34 samples tested positive for IFA-immunoglobulin G (IgG) and IFA-IgM, respectively. By using Youden's index, the cutoff values of ELISA-IgG at 0.915 gave a sensitivity of 84.5% and specificity of 94%. The cutoff values of ELISA-IgM at 0.634 gave a sensitivity of 88.2% and specificity of 85.1%. Using latent class analysis, estimates of sensitivity and specificity of almost all the assays were slightly higher than those of a conventional method of calculation. The test is proved beneficial for discriminating between infected and non-infected individuals with the advantage of low-cost and high-throughput capability.

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Conflict of interest statement

Disclosure: CCC and WMC are employees of the U. S. Government. This work was prepared as part of their official duties. Title 17 U.S.C. §101 defines a U.S. Government work as a work prepared by an employee of the U.S. Government as part of that person's official duties, and “copyright protection” under this title is not available for any work of the United States Government.

Figures

Figure 1.
Figure 1.
Scatterplot analysis of 302 sera measured by enzyme-linked immunosorbent assay (ELISA)-immunoglobulin G (IgG) using rPap31 antigen. The vertical line separates immunofluorescent assay (IFA)-IgG-positive sera (sera nos. 1 to 103) from negative sera (sera nos. 104 to 302). The horizontal line indicates the cutoff value of the ELISA-IgG assay.
Figure 2.
Figure 2.
Scatterplot analysis of 302 sera measured by enzyme-linked immunosorbent assay (ELISA)-immunoglobulin M (IgM) using rPap31 antigen. The vertical line separates immunofluorescent assay (IFA)-IgM-positive sera (sera nos. 1 to 34) from negative sera (sera nos. 35 to 302). The horizontal line indicates the cutoff value of the ELISA-IgM assay.
Figure 3.
Figure 3.
Receiver operating characteristics (ROC) curves of rPap31-based enzyme-linked immunosorbent assay (ELISA) for detection of antibody against Bartonella bacilliformis. (A) Shows the ROC curve of ELISA-immunoglobulin G (IgG) with an area under the ROC curve (AUC) of 0.95 (95% confidence interval [CI] = 0.93–0.97). (B) Shows the ROC curve of ELISA-IgM with an AUC of 0.93 (95% CI = 0.89–0.97).
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