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. 2013 Apr;7(1):49-56.
Epub 2013 Mar 6.

Effect of purine nucleoside analogue-acyclovir on the sperm parameters and testosterone production in rats

Elham Movahed  1 , Rajabali Sadrkhanlou  2 , Abbas Ahmadi  2 , Vahid Nejati  1 , Zahra Zamani  1
Affiliations

Effect of purine nucleoside analogue-acyclovir on the sperm parameters and testosterone production in rats

Elham Movahed et al. Int J Fertil Steril. 2013 Apr.

Abstract

Background: Acyclovir (ACV), a synthetic purine nucleoside analogue derived from guanosine, is known to be toxic to gonads and the aim of this study was to evaluate the effect of ACV on the sperm parameters and testosterone production in rat.

Materials and methods: In this experimental study, forty adult male Wistar rats (220 ± 20 g) were randomly divided into five groups (n=8 for each group). One group served as control and one group served as sham control [distilled water was intraperitoneally (i.p.) injected]. ACV was administered intraperitoneally in the drug treatment groups (4, 16 and 48 mg/kg/day) for 15 days. Eighteen days after the last injection, rats were sacrificed by CO2 inhalation. After that, cauda epididymides were removed surgically. At the end, sperm concentrations in the cauda epididymis, sperm motility, morphology, viability, chromatin quality and DNA integrity were analyzed. Serum testosterone concentrations were determined.

Results: The results showed that ACV did not affect sperm count, but decreased sperm motility and sperm viability at 16 and 48 mg/kg dose-levels. Sperm abnormalities increased at 48 mg/kg dose-level of ACV. Further, ACV significantly increases DNA damage at 16 and 48 mg/kg dose-levels and chromatin abnormality at all doses. Besides, a significant decrease in serum testosterone concentrations was observed at 16 and 48 mg/ kg doses.

Conclusion: The present results highly support the idea that ACV induces testicular toxicity by adverse effects on the sperm parameters and serum level of testosterone in male rats.

Keywords: Acyclovir; Antiviral Drugs; Rat; Sperm Parameters; Testosterone.

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Figures

Fig 1
Fig 1
Dead sperm appear pink and live sperm are not stained, Eosin/Nigrosinstaining technique (×2000).
Fig 2
Fig 2
A. Sperm with damaged DNA (yellow), B. Sperm with normal DNA (green). Acridine-orange stainingtechnique (×2000).
Fig 3
Fig 3
Sperm head containing immature nuclear chromatin is dark blue and sperm head with mature nuclei is light blue. Aniline blue staining technique (×2000).
Fig 4
Fig 4
Effects of ACV on serum testosterone concentrations in adult male rats. 4 rats from each group were analysedin this experiment. Error bars indicate the standard error of the mean. *; p<0.05 and **; p<0.01. a; Significant compared with control, b; Significant compared with sham control and c; Significant compared with 4 mg/kg ACV.
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