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. 2014 Feb 11;15(2):2454-64.
doi: 10.3390/ijms15022454.

Evaluation of osseointegration of titanium alloyed implants modified by plasma polymerization

Carolin Gabler  1 , Carmen Zietz  2 , Rebecca Göhler  3 , Andreas Fritsche  4 , Tobias Lindner  5 , Maximilian Haenle  6 , Birgit Finke  7 , Jürgen Meichsner  8 , Solvig Lenz  9 , Bernhard Frerich  10 , Frank Lüthen  11 , J Barbara Nebe  12 , Rainer Bader  13
Affiliations

Evaluation of osseointegration of titanium alloyed implants modified by plasma polymerization

Carolin Gabler et al. Int J Mol Sci. .

Abstract

By means of plasma polymerization, positively charged, nanometre-thin coatings can be applied to implant surfaces. The aim of the present study was to quantify the adhesion of human bone cells in vitro and to evaluate the bone ongrowth in vivo, on titanium surfaces modified by plasma polymer coatings. Different implant surface configurations were examined: titanium alloy (Ti6Al4V) coated with plasma-polymerized allylamine (PPAAm) and plasma-polymerized ethylenediamine (PPEDA) versus uncoated. Shear stress on human osteoblast-like MG-63 cells was investigated in vitro using a spinning disc device. Furthermore, bone-to-implant contact (BIC) was evaluated in vivo. Custom-made conical titanium implants were inserted at the medial tibia of female Sprague-Dawley rats. After a follow-up of six weeks, the BIC was determined by means of histomorphometry. The quantification of cell adhesion showed a significantly higher shear stress for MG-63 cells on PPAAm and PPEDA compared to uncoated Ti6Al4V. Uncoated titanium alloyed implants showed the lowest BIC (40.4%). Implants with PPAAm coating revealed a clear but not significant increase of the BIC (58.5%) and implants with PPEDA a significantly increased BIC (63.7%). In conclusion, plasma polymer coatings demonstrate enhanced cell adhesion and bone ongrowth compared to uncoated titanium surfaces.

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Figures

Figure 1.
Figure 1.
Shear stress (N/m2) exerted on MG-63 cells deployed on different implant surface configurations (uncoated, PPAAm-coated and PPEDA-coated titanium discs). Shear stress on plasma polymer coatings was significantly higher compared to uncoated Ti6Al4V (* p ≤ 0.05; ** p ≤ 0.001).
Figure 2.
Figure 2.
Bone-to-implant contact (BIC) (%) of the implants with different titanium implant surface configurations (uncoated, PPAAm-coated and PPEDA-coated) after six weeks in vivo. BIC was increased by plasma polymer coatings (* p ≤ 0.05).
Figure 3.
Figure 3.
(a) Implant for insertion in the tibial metaphysis of the Sprague-Dawley rats; (b) Retrieved tibial bone with an implant in the medial metaphysis.
Figure 4.
Figure 4.
Histomorphometric sample with a PPAAm-coated implant in the proximal rat tibia (toluidine blue staining). Green line marks the evaluated implant region for BIC.
See this image and copyright information in PMC

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