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. 1988 Jun;62(6):2200-3.
doi: 10.1128/JVI.62.6.2200-2203.1988.

Mapping of monoclonal antibodies to the Sendai virus P protein and the location of its phosphates

S Vidal  1 , J Curran, C Orvell, D Kolakofsky
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Mapping of monoclonal antibodies to the Sendai virus P protein and the location of its phosphates

S Vidal et al. J Virol. 1988 Jun.

Abstract

The epitopes of a panel of five monoclonal antibodies to the Sendai virus P protein were mapped by generating modified forms of the P mRNA via SP6 expression followed by in vitro translation. The epitopes were found to be clustered in the C-terminal region of the protein. Two epitopes were within the last 30 residues, two were within the next 65, and one was between residues 308 and 451 of this 568-residue-long protein. By a combination of partial proteolysis and Western immunoblotting with one of these antibodies, the sites at which phosphates are added in vitro by the virion-associated kinase were mapped to the second quarter of the molecule from the N terminus.

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