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. 1988 May;85(10):3555-9.
doi: 10.1073/pnas.85.10.3555.

Glycolipid reanchoring of T-lymphocyte surface antigen CD8 using the 3' end sequence of decay-accelerating factor's mRNA

M L Tykocinski  1 , H K Shu, D J Ayers, E I Walter, R R Getty, R K Groger, C A Hauer, M E Medof
Affiliations

Glycolipid reanchoring of T-lymphocyte surface antigen CD8 using the 3' end sequence of decay-accelerating factor's mRNA

M L Tykocinski et al. Proc Natl Acad Sci U S A. 1988 May.

Abstract

Decay-accelerating factor (DAF) is one of a family of cell-associated proteins that undergo posttranslational modifications in which glycolipid anchoring structures are substituted for membrane-spanning sequences. The signals that direct the covalent substitution reaction in these proteins are unknown. Human DAF was expressed in Chinese hamster ovary (CHO) cells and murine BW lymphocytes. In both cases, the xenogeneic DAF in transfectants incorporated a glycolipid anchor. A chimeric CD8-DAF cDNA, encompassing the extra-cellular region of the T-lymphocyte surface antigen CD8 and the 3' end of DAF mRNA (encoding the C-terminal region of mature DAF as well as the hydrophobic extension peptide), was expressed in human leukemia lines after transfection with an Epstein-Barr virus-based episomal vector. The chimeric protein in transfectants demonstrated glycolipid anchoring, whereas unaltered CD8 in control experiments did not. The signals directing glycolipid anchoring in eukaryotic cells are thus evolutionarily conserved and contained in the 3' end of the DAF sequence.

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References

    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Nucleic Acids Res. 1987 Feb 11;15(3):1311-26 - PubMed
    1. Neurosci Lett. 1983 Sep 19;40(1):85-9 - PubMed
    1. J Exp Med. 1984 Nov 1;160(5):1558-78 - PubMed
    1. Cell. 1985 Feb;40(2):237-46 - PubMed

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