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. 2014 Jan;26(1):141-5.
doi: 10.1177/1040638713517233.

Coxiella burnetii serology assays in goat abortion storm

Michelle P Emery  1 , Eileen N Ostlund, Mohamed Ait Ichou, Jeff D Ballin, David McFarling, Luanne McGonigle
Affiliations

Coxiella burnetii serology assays in goat abortion storm

Michelle P Emery et al. J Vet Diagn Invest. 2014 Jan.

Abstract

Many commercial antibody detection enzyme-linked immunosorbent assay (ELISA) kits for Q fever utilize the Nine Mile (Montana tick) strain of Coxiella burnetii as antigen. An ELISA kit manufactured in France employs ovine placenta-sourced antigen and has been used in Europe. Sera from goats experiencing a Q fever abortion storm in the United States were used to compare the sensitivity and specificity of these 2 ELISA formats and the Q fever complement fixation test (CFT). Latent class estimates of sensitivity ranged from 97% to 100% with a specificity of 95-100% for the 2 ELISA kits. Estimates for sensitivity and specificity of the CFT were 89% and 82%, respectively. There was not a significant increase in ELISA sensitivity observed with the ovine-sourced antigen kit in this study. Real-time polymerase chain reactions performed on a portion of the sera found that 15 out of 20 sera were congruent across 4 tests for positive and negative sera.

Keywords: Abortion storm; Coxiella burnetii; Nine Mile; ovine enzyme-linked immunosorbent assay; polymerase chain reaction.

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