Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:21:6.
doi: 10.1051/parasite/2014007. Epub 2014 Feb 19.

Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy

Giovanna Chessa  1 , Valentina Chisu  1 , Rosaura Porcu  1 , Giovanna Masala  1
Affiliations

Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy

Giovanna Chessa et al. Parasite. 2014.

Abstract
in English, French

During 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5'-SAG2, 3'-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.

Pendant la période 2009–2010, 161 échantillons de tissus (142 placentas, 16 cervelles et 3 foies) provenant de fœtus ovins avortés de Sardaigne, Italie, ont été testés pour la toxoplasmose. Les organes trouvés positifs par PCR visant la région ITS1 de Toxoplasma gondii ont été amplifiés avec 11 marqueurs génétiques (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 et Apico) et ensuite soumis à PCR/RFLP pour génotypage. De l’ADN de T. gondii a été trouvé dans 5 placentas, 14 cervelles et 2 foies par les analyses PCR et tous les isolats avaient des allèles de Type II pour les 11 loci avec tous les 11 marqueurs. Les résultats indiquent que T. gondii de Type II est associé avec les avortements ovins.

PubMed Disclaimer

References

    1. Dubey JP, Sundar N, Hill D, Velmurugan GV, Bandini LA, Kwok OC, Majumdar D, Su C. 2008. High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA. International Journal for Parasitology, 38, 999–1006 - PubMed
    1. Edwards JF, Dubey JP. 2013. Toxoplasma gondii abortion storm in sheep on a Texas farm and isolation of mouse virulent atypical genotype T. gondii from an aborted lamb from a chronically infected ewe. Veterinary Parasitology, 192, 129–136 - PubMed
    1. Dumètre A, Ajzenberg D, Rozette L, Mercier A, Dardé ML. 2006. Toxoplasma gondii infection in sheep from Haute-Vienne, France: seroprevalence and isolate genotyping by microsatellite analysis. Veterinary Parasitology, 142, 376–379 - PubMed
    1. Faull WB, Clarkson MJ, Winter AC. 1986. Toxoplasmosis in a flock of sheep: some investigations into its source and control. Veterinary Record, 119, 491–493 - PubMed
    1. Halos L, Thébault A, Aubert D, Thomas M, Perret C, Geers R, Alliot A, Escotte-Binet S, Ajzenberg D, Dardé ML, Durand B, Boireau P, Villena I. 2010. An innovative survey underlining the significant level of contamination by Toxoplasma gondii of ovine meat consumed in France. International Journal for Parasitology, 40, 193–200 - PubMed

MeSH terms

Substances

LinkOut - more resources