Studies of the early stages of optic axon regeneration in the goldfish

Abstract

We have studied the early stages (4-14 days) of axonal regeneration following intraorbital optic nerve crush in the goldfish. We used 3H-proline autoradiography to anterogradely label and visualize the growing axons and wheat germ agglutinin-conjugated horseradish peroxidase (WGA:HRP) for retrograde labeling to determine the cells of origin of the earliest projections. The first retinal ganglion cells (RGCs) that could be retrogradely filled from the optic tract, following optic nerve crush, were in the central retina and were seen at 8 days postoperative. More peripheral cells were only labeled with longer postcrush survival periods. Thus, the first axons to regenerate past the lesion were from central RGCs. The axons of these cells extended into the cranial nerve stump between 4 and 5 days postcrush and entered the nerve as a fascicle, which travelled just beneath its surface. Studies of nerve cross sections from animals at 5-8 days postoperative demonstrated that initial outgrowth was not confined to any particular locale within the nerve although the early fibers appeared to avoid its temporal aspect. When the regenerating axons reached the optic tract they remained in fascicles but left the surface to run along the medial, deep portion of the tract, immediately adjacent to the diencephalon and pretectum. The positions occupied by the earliest-regenerating axons in the optic nerve were variable and not always appropriate for their central retinal origin. However, the abrupt change in growth trajectory as the fibers entered the optic tract brought them into the areas of the visual paths that are occupied by central axons in intact animals. We suggest that this change in position is related to both changes in the structural organization of the intracranial visual paths and to possible axon guidance signals in the region of the nerve-tract juncture.