Gibberellin-like substances in peach tissue: Separation by thin-layer electrophoresis and chromatography

Abstract

Thin-layer electrophoresis in conjunction with thin-layer chromatography proved an excellent method for separating gibberellin-like compounds extracted from small quantities of peach tissue. Eleven compounds active in the barley-endosperm gibberellin bioassay were separated from peach shoot tips. Two of these were possibly identical with known gibberellins but the remainder were distinctly different. Of the latter, five were acidic, one was neutral, and three were basic. The most active compound in shoot tips may have been GA1 or GA3 but in seeds the most active compound resembled no known gibberellin.