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. 2014 Feb 13;9(2):e87023.
doi: 10.1371/journal.pone.0087023. eCollection 2014.

Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study

Francisco Mesa  1 , Francisco O'Valle  2 , Manfredi Rizzo  3 , Francesco Cappello  4 , Nikos Donos  5 , Mohamed Parkar  5 , Navidah Chaudhary  5 , Francesco Carini  6 , Ricardo Muñoz  7 , Luigi Nibali  5
Affiliations

Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study

Francisco Mesa et al. PLoS One. .

Abstract

Objectives: The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.

Design: A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.

Results: The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).

Conclusions: COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Panoramic microphotograph of gingival biopsies.
A) Gingival health group; B) Gingivitis; C) Chronic periodontitis. (Hematoxylin-eosin, original magnification ×2). (Bar 1000 µm).
Figure 2
Figure 2. Gingival epithelium and lamina propria with variable chronic inflammatory infiltrate.
A) Mild infiltrate in gingival health group; B) mild/moderate infiltrate in gingivitis; and C) severe infiltrate in sulcular epithelium in chronic periodontitis (Hematoxylin-eosin, original magnification ×10). (Bar 100 µm).
Figure 3
Figure 3. Representative microphotograph of COX-2 immunohistochemical expression (brown-stained) in gingival biopsies.
A) Gingival health group; B) Gingivitis; C) Chronic periodontitis (Polymer-peroxidase-based method, original magnification ×10).
Figure 4
Figure 4. Representative microphotograph of IL-6 immunohistochemical expression (brown-stained) in gingival biopsies.
A) Gingival health group; B) Gingivitis; C) Chronic periodontitis (original magnification ×40) (Bar 100 µm).
See this image and copyright information in PMC

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