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. 2013 Winter;2(1):9-13.

Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

Seyed Mahmoud Amin Marashi  1 , Ramazan Rajabnia  2 , Abbas Ali Imani Fooladi  3 , Zohreh Hojati  4 , Sharareh Moghim  5 , Bahram Nasr Esfahani  5
Affiliations

Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

Seyed Mahmoud Amin Marashi et al. Int J Mol Cell Med. 2013 Winter.

Abstract

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.

Keywords: RT-qPCR; Vibrio cholerae; ctxAB expression.

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Figures

Fig 1
Fig 1
Diagram of the vibrio cholerae ToxR regulon and ctxAB expression, with permission from ASM
Fig 2
Fig 2
Melting curves of ctxAB and recA genes
Fig 3
Fig 3
Level of cholera toxin production in classical and EL Tor strains
See this image and copyright information in PMC

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