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. 1966 Jun;72(2):155-61.
doi: 10.1007/BF00387479.

The bioassay of gibberellins

R L Jones  1 , J E Varner
Affiliations

The bioassay of gibberellins

R L Jones et al. Planta. 1966 Jun.

Abstract

A bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: 1) Release of α-amylase is one step closer to the primary site of action of GA; 2) release of α-amylase is not affected by solvent residues and is apparently completely specific for gibberellin; 3) release of α-amylase is not affected by substances other than gibberellins present in crude plant extracts.

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References

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