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. 2014 Feb 17;24(4):R148-9.
doi: 10.1016/j.cub.2014.01.019.

The mitotic origin of chromosomal instability

Samuel F Bakhoum  1 , William T Silkworth  2 , Isaac K Nardi  3 , Joshua M Nicholson  4 , Duane A Compton  5 , Daniela Cimini  6
Affiliations

The mitotic origin of chromosomal instability

Samuel F Bakhoum et al. Curr Biol. .
No abstract available

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Figures

Figure 1
Figure 1
Mitotic errors cause chromosome instability(Au: OK?). (A) Model of whole chromosome a neuploidy arising via formation of a merotelically attached (kMTs to both poles) lagging chromosome (LC) in mitosis. (B) Percent of anaphase cells with lagging chromosomes, chromatin bridges (CBs) or acentric fragments (ACs) in CIN− (HCT116 and DLD1) and CIN+ (HT-29, SW620, MCF-7, HeLa) cells. Data are represented as mean ± s.e.m., n = 448–1316 cells in 3–6 independent experiments. χ2, p<0.0001 for lagging chromosomes between CIN− and CIN+ cells.(C) Percent of chromosome spreads containing intact chromosomes or chromosome fragments in HCT116 (CIN−) and U251 (CIN+) cells, n = >300 spreads, p=0.26, χ2-test. (D) Percent of anaphase cells with lagging chromosomes in control and GFP–Kif2b over-expressing U251 and U118 cells. Data are reported as mean ± s.e.m., n = 150 cells. (E–F) Percent deviation from the modal chromosome number (noted above the bars) for the given chromosomes in a clonogenic assay (30 days) in CIN+ U251 and U118 control and GFP–Kif2b-overexpressing cells, n = 300 cells.
See this image and copyright information in PMC

Comment in

  • Response to Bakhoum et al.
    Burrell RA, McClelland SE, Bartek J, Swanton C. Burrell RA, et al. Curr Biol. 2014 Feb 17;24(4):R150. doi: 10.1016/j.cub.2014.01.021. Curr Biol. 2014. PMID: 24556434 No abstract available.

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