[Variations in the protein and enzyme pattern during pollen meiosis and pollen development]

Abstract

During pollen development and meiosis in Lilium henryi the protein pattern was analysed by disc electrophoresis, the microsporocytes and the tapetal fractions, being taken seperately. The pattern undergoes a phase-specific variation: every cytological stage shows a specific protein pattern.The fractions could be partially identified by incubation with substrate and by specific enzyme staining of some of the enzymes (phosphorylase, acid phosphatase, peroxydase, thiamine pyrophosphatase, malate dehydrogenase, lactate dehydrogenase). The enzyme pattern also has a high phase specificity. Iso-enzymes were found in various numbers and activity, especially in the case of peroxydase and lactat dehydrogenase. The results are interpreted as a series of phase-specific gene transcriptions which coordinate the sequence of biochemical events essential for the normal pollen meiosis and the subsequent development of the pollen.