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. 1988 Aug;19(1-2):159-65.
doi: 10.1016/0165-5728(88)90045-8.

Cytotoxic effect of myelin basic protein-reactive T cells on cultured oligodendrocytes

K Kawai  1 , B Zweiman
Affiliations

Cytotoxic effect of myelin basic protein-reactive T cells on cultured oligodendrocytes

K Kawai et al. J Neuroimmunol. 1988 Aug.

Abstract

To help clarify effector mechanisms in experimental allergic encephalitis (EAE), the cytotoxic effects of myelin basic protein (MBP)-reactive lymphocytes on oligodendrocytes were studied using a 51Cr release assay. MBP-reactive encephalitogenic T cell lines were cytotoxic to 51Cr-labeled oligodendrocyte target cells derived from Lewis rat fetal brain-dissociated culture, when incubated for 6 h in the presence of antigen-presenting cells (APC) and MBP (percent 51Cr release = 65 +/- 3% vs. spontaneous release = 22 +/- 3% vs. normal lymph node cells + APC and MBP = 20 +/- 3%). This reaction is time dependent, likely MHC restricted, and is not just a nonspecific toxic effect against any Lewis target cells since neither fibroblasts nor astrocytes were affected. Other (tetanus toxoid-reactive) lymphoblasts stimulated by specific antigen were not cytotoxic to the oligodendrocytes. These findings suggest that oligodendrocytes might be target cells for MBP-reactive lymphocytes in EAE if antigen presentation is appropriate.

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