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. 2014 Jan 23;5(2):143-50.
doi: 10.7150/jca.7439. eCollection 2014.

Evaluation of antioxidant and antiangiogenic properties of caesalpinia echinata extracts

Elisangela Christhianne Barbosa da Silva Gomes  1 , George Chaves Jimenez  2 , Luis Claudio Nascimento da Silva  3 , Fabrício Bezerra de Sá  4 , Karen Pena Cavalcanti de Souza  5 , Gerson S Paiva  6 , Ivone Antônia de Souza  7
Affiliations

Evaluation of antioxidant and antiangiogenic properties of caesalpinia echinata extracts

Elisangela Christhianne Barbosa da Silva Gomes et al. J Cancer. .

Abstract

Natural products contain important combinations of ingredients, which may to some extent help to modulate the effects produced by oxidation substrates in biological systems. It is known that substances capable of modulating the action of these oxidants on tissue may be important allies in the control of neovascularization in pathological processes. The aim of this study was to evaluate the antioxidant and antiangiogenic properties of an ethanol extract of Caesalpinia echinata. The evaluation of antioxidant properties was tested using two methods (DPPH inhibition and sequestration of nitric oxide). The antiangiogenic properties were evaluated using the inflammatory angiogenesis model in the corneas of rats. The extract of C. echinata demonstrated a high capacity to inhibit free radicals, with IC50 equal to 42.404 µg/mL for the DPPH test and 234.2 µg/mL for nitric oxide. Moreover, it showed itself capable of inhibiting the inflammatory angiogenic response by 77.49%. These data suggest that biochemical components belonging to the extract of C. echinata interfere in mechanisms that control the angiogenic process, mediated by substrates belonging to the arachidonic acid cascade, although the data described above also suggest that the NO buffer may contribute to some extent to the reduction in the angiogenic response.

Keywords: Caesalpinia echinata; angiogenesis; antioxidant; natural products.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
Inhibition of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by ethanol extract of Caesalpinia echinata. Significant values ± S.D. (n= 3)
Figure 2
Figure 2
Antioxidant activity of brazilin
Figure 3
Figure 3
Inhibition of NO radical by the ethanol extract of Caesalpinia echinata. Significant values ± S.D. (n= 3)
Figure 4
Figure 4
Viability of culture of VERO cells subjected to different concentrations of the ethanolic extract of Caesalpinia echinata.
Figure 5
Figure 5
A) Overview of crystalline rats stimulated with NaOH (at the time of cauterizing); B) View of crystal in Wistar rat stimulated with NaOH, not treated (control); C) crystal in Wistar rat treated with EECe.
Figure 6
Figure 6
Number of vessels counted on the surface of the crystal in Wistar rats treated with EECe.
See this image and copyright information in PMC

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