Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2014 Mar 11;111(10):3659-60.
doi: 10.1073/pnas.1401325111. Epub 2014 Feb 24.

Examining how enzymes self-organize in a membrane

Thomas C Pochapsky  1
Affiliations
Comment

Examining how enzymes self-organize in a membrane

Thomas C Pochapsky. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Three-domain structure of CYP51 from S. cerevisiae as described in ref. (PDB code 4K0F). The ribbon diagram is rainbow coded (N-terminal, blue; C-terminal, red). An N-terminal AH is predicted to bind to the inner surface of the ER membrane in vivo and is connected to the CYP on the outer membrane surface via a TMH. Positions of some secondary structural features in the CYP domain, including the active site heme and the F, G, and I helices, are noted. Parallel black lines indicate approximate position proposed for the lipid bilayer. Graphics were generated using PyMOL (11).
Fig. 2.
Fig. 2.
Residues involved in polar interactions between the TMH and CYP domains of lanosterol 14α demethylase (CYP51) from S. cerevisiae (PDB code 4K0F).
Fig. 3.
Fig. 3.
Alignment of membrane-interacting regions of two CYP51 structures: one from Monk et al. (4) (PDB code 4K0F) in magenta and that of the truncated T. cruzi enzyme (10) (PDB code 3K1O) in cyan. The region interacting with the TMH in 4K0F (near the A helix and β1 sheet) is shown for both enzymes. The loop connecting the F and G helices in 3K1O is disordered in the T. cruzi enzyme between Pro-216 and Leu-221, as is the loop following the G helix, between Lys-254 and Asn-257. The structure is ordered in both places in the 4K0F structure.
See this image and copyright information in PMC

Comment on

References

    1. Myers WK, Lee YT, Britt RD, Goodin DB. The conformation of P450cam in complex with putidaredoxin is dependent on oxidation state. J Am Chem Soc. 2013;135(32):11732–11735. - PMC - PubMed
    1. Asciutto EK, Young MJ, Madura J, Pochapsky SS, Pochapsky TC. Solution structural ensembles of substrate-free cytochrome P450(cam) Biochemistry. 2012;51(16):3383–3393. - PMC - PubMed
    1. Labeikovsky W, Eisenmesser EZ, Bosco DA, Kern D. Structure and dynamics of pin1 during catalysis by NMR. J Mol Biol. 2007;367(5):1370–1381. - PMC - PubMed
    1. Monk BC, et al. Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer. Proc Natl Acad Sci USA. 2014;111:3865–3870. - PMC - PubMed
    1. Pochapsky TC, Kazanis S, Dang M. Conformational plasticity and structure/function relationships in cytochromes P450. Antioxid Redox Signal. 2010;13(8):1273–1296. - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources