Environmental effects on molecular and phenotypic variation in populations of Eruca sativa across a steep climatic gradient

Abstract

In Israel Eruca sativa has a geographically narrow distribution across a steep climatic gradient that ranges from mesic Mediterranean to hot desert environments. These conditions offer an opportunity to study the influence of the environment on intraspecific genetic variation. For this, we combined an analysis of neutral genetic markers with a phenotypic evaluation in common-garden experiments, and environmental characterization of populations that included climatic and edaphic parameters, as well as geographic distribution. A Bayesian clustering of individuals from nine representative populations based on amplified fragment length polymorphism (AFLP) divided the populations into a southern and a northern geographic cluster, with one admixed population at the geographic border between them. Linear mixed models, with cluster added as a grouping factor, revealed no clear effects of environment or geography on genetic distances, but this may be due to a strong association of geography and environment with genetic clusters. However, environmental factors accounted for part of the phenotypic variation observed in the common-garden experiments. In addition, candidate loci for selection were identified by association with environmental parameters and by two outlier methods. One locus, identified by all three methods, also showed an association with trichome density and herbivore damage, in net-house and field experiments, respectively. Accordingly, we propose that because trichomes are directly linked to defense against both herbivores and excess radiation, they could potentially be related to adaptive variation in these populations. These results demonstrate the value of combining environmental and phenotypic data with a detailed genetic survey when studying adaptation in plant populations. This article describes the use of several types of data to estimate the influence of the environment on intraspecific genetic variation in populations originating from a steep climatic gradient. In addition to molecular marker data, we made use of phenotypic evaluation from common garden experiments, and a broad GIS based environmental data with edaphic information gathered in the field. This study, among others, lead to the identification of an outlier locus with an association to trichome formation and herbivore defense, and its ecological adaptive value is discussed.

Keywords: Environmental adaptation; Eruca sativa; genetic diversity; outlier loci; phenotypic variation.

Figure 1

A population of Eruca sativa in the Jordan Valley desert habitat.

Figure 2

Locations of Eruca sativa populations and of Agricultural Research Organization where phenotypic evaluation was performed in common-garden and net-house experiments.

Figure 3

Inferred clusters of individual genotypes and admixture results for MZ, as calculated with BAPS software, for populations of Eruca sativa. Each vertical bar represents one individual genotype. Individuals with two colors have admixed genotypes from the two main southern and northern genotype clusters. The admixture analysis was done with the eight unmixed populations used as ancestral gene pools.