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. 1988 Sep;62(9):3109-19.
doi: 10.1128/JVI.62.9.3109-3119.1988.

Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells

D R O'Reilly  1 , L K Miller
Affiliations

Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells

D R O'Reilly et al. J Virol. 1988 Sep.

Abstract

Recombinant baculoviruses were constructed which express simian virus 40 large T antigen (SVT-Ag) or murine p53 to high levels in infected insect cells. Characterization of the expressed proteins revealed that they display many properties of the corresponding mammalian-derived proteins. Both proteins are of wild-type size, localize to the nucleus, are recognized by several SVT-Ag- or p53-specific monoclonal antibodies, and are phosphorylated in this system. Complexes are formed between baculovirus-derived SVT-Ag and p53 after coinfection of insect cells with both recombinant viruses. After infection of insect cells with either virus individually, each protein can self-associate to form a variety of oligomeric species. Pulse-chase experiments indicated that both SVT-Ag and p53 are highly stable in insect cells, even in the absence of complex formation.

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