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. 2014 Feb 26;3(1):e000792.
doi: 10.1161/JAHA.113.000792.

Circulating microRNAs: understanding the limits for quantitative measurement by real-time PCR

Anandwardhan A Hardikar  1 , Ryan J Farr, Mugdha V Joglekar
Affiliations

Circulating microRNAs: understanding the limits for quantitative measurement by real-time PCR

Anandwardhan A Hardikar et al. J Am Heart Assoc. .
No abstract available

Keywords: biomarker; circulation; diagnosis; microRNA.

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Figures

Figure 1.
Figure 1.
Two theoretical data sets (A and B) plotted as raw data/cycle‐threshold (Ct) values (left panel), fold over control (center panel) and fold over detectable (right panel). See text for details on the Data Presentation methods and flaws in clarity of presentation for the most popular (fold‐over‐control) method.
See this image and copyright information in PMC

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References

    1. Sato F, Tsuchiya S, Terasawa K, Tsujimoto G. Intra‐platform repeatability and inter‐platform comparability of microRNA microarray technology. PLoS One. 2009; 4:e5540. - PMC - PubMed
    1. Jensen SG, Lamy P, Rasmussen MH, Ostenfeld MS, Dyrskjot L, Orntoft TF, Andersen CL. Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs. BMC Genomics. 2011; 12:435. - PMC - PubMed
    1. Git A, Dvinge H, Salmon‐Divon M, Osborne M, Kutter C, Hadfield J, Bertone P, Caldas C. Systematic comparison of microarray profiling, real‐time PCR, and next‐generation sequencing technologies for measuring differential microRNA expression. RNA. 2010; 16:991-1006 - PMC - PubMed
    1. Karlsen F, Steen HB, Nesland JM. SYBR green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. J Virol Methods. 1995; 55:153-156 - PubMed
    1. Schneeberger C, Speiser P, Kury F, Zeillinger R. Quantitative detection of reverse transcriptase‐PCR products by means of a novel and sensitive DNA stain. PCR Methods Appl. 1995; 4:234-238 - PubMed

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