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. 2014 May;52(5):1737-40.
doi: 10.1128/JCM.00328-14. Epub 2014 Feb 26.

Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory

M J Buitrago  1 , L Bernal-Martinez, M V Castelli, J L Rodriguez-Tudela, M Cuenca-Estrella
Affiliations

Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory

M J Buitrago et al. J Clin Microbiol. 2014 May.

Abstract

A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.

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Figures

FIG 1
FIG 1
Schematic diagram of procedures performed with biopsy samples, depending on clinical suspicion. When specific assay results were negative, a panfungal assay was performed. *, specific assays detected the following species: A. fumigatus, A. terreus, A. flavus, Histoplasma capsulatum, Paracoccidioides brasiliensis, Rhizopus oryzae, Rhizopus microsporus, Mucor spp., Fusarium spp., Scedosporium prolificans, and Scedosporium apiospermum.
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